Publications/業績


Journal articles (since 2000)

A. Hayashi, R. Hemmi, Y. Saito, R. Utoh, T. Taniguchi, and M. Yamada: High-density Microporous Drainage-integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting Systems, Analytical Chemistry, in press (2024).


S. Mashiyama, R. Hemmi, T. Sato, A. Kato, T. Taniguchi, and M. Yamada: Pushing the Limits of Microfluidic Droplet Production Efficiency: Engineering Microchannels with Seamlessly Implemented 3D Inverse Colloidal Crystals, Lab on a Chip, 24, 171–181 (2024). (highlighted as outside front cover)
  (linked to the website)

M. Takagi, M. Yamada, R. Utoh, and M. Seki: A Multiscale, Vertical-flow Perfusion System with Integrated Porous Microchambers for Upgrading Multicellular Spheroid Culture, Lab on a Chip, 23, 2257–2267 (2023).


Y. Hasebe, M. Yamada, R. Utoh, and M. Seki: Expansion of CHO Cells via a Loose Cluster-assisted Suspension Culture Using Cell-sized Gelatin Microcarriers, Journal of Bioscience and Bioengineering, 135, 417–422 (2023).


N. Shimmyo, M. Furuhata, M. Yamada, R. Utoh, and M. Seki: Process Simplification and Structure Design of Parallelized Microslit Isolator for Physical Property-based Capture of Tumor Cells, Analyst, 147, 1622–1630 (2022).


A. Morita, M. Yamada, R. Utoh, K. Momiyama, H. Iwadate, and M. Seki: Formation of 3D Tissues of Primary Hepatocytes Using Fibrillized Collagen Microparticles as Intercellular Binders, Journal of Bioscience and Bioengineering, 133, 265–272 (2022).


R. Utoh, S. Enomoto, M. Yamada, K. Yamanaka, Y. Yajima, K. Furusawa, and M. Seki: Polyanion-induced, Microfluidic Engineering of Fragmented Collagen Microfibers for Reconstituting Extracellular Environments of 3D Hepatocyte Culture, Materials Science & Engineering C, 129, 112417 (2021).


K. Saeki, H. Hiramatsu, A. Hori, Y. Hirai, M. Yamada, R. Utoh, and M. Seki: Sacrificial Alginate-assisted Microfluidic Engineering of Cell-supportive Protein Microfibers for Hydrogel-based Cell Encapsulation, ACS Omega, 5 (34), 21641–21650 (2020).


T. Mu, H. Toyoda, Y. Kimura, M. Yamada, R. Utoh, D. Umeno, and M. Seki: Laborless, Automated Microfluidic Tandem Cell Processor for Visualizing Intracellular Molecules of Mammalian Cells, Analytical Chemistry, 92 (3), 2580–2588 (2020). (highlighted as front cover)
  (linked to the website)

S. Aoyama, K. Monden, Y. Akiyama, M. Yamada, and M. Seki: Enhanced Immunoadsorption on Imprinted Polymeric Microstructures with Nanoengineered Surface Topography for Lateral Flow Immunoassay Systems, Analytical Chemistry, 91 (21), 13377–13382 (2019).


T. Yanai, T. Ouchi, M. Yamada, and M. Seki: Hydrodynamic Microparticle Separation Mechanism Using Three-dimensional Flow Profiles in Dual-depth and Asymmetric Lattice-shaped Microchannel Networks, Micromachines, 10 (6), 425 (2019).


A. Hori, Y. Watabe, M. Yamada, Y. Yajima, R. Utoh, and M. Seki: One-step Formation of Microporous Hydrogel Sponges Encapsulating Living Cells by Utilizing Bicontinuous Dispersion of Aqueous Polymer Solutions, ACS Applied Bio Materials, 2 (5), 2237–2245 (2019). (highlighted as cover)
  (linked to the website)

R. Ozawa, H. Iwadate, H. Toyoda, M. Yamada, and M. Seki: A Numbering-up Strategy of Hydrodynamic Microfluidic Filters for Continuous-flow High-throughput Cell Sorting, Lab on a Chip, 19 (10), 1828–1837 (2019).


M. Fukushi, K. Kinoshita, M. Yamada, Y. Yajima, R. Utoh, and M. Seki: Formation of Pressurizable Hydrogel-based Vascular Tissue Models by Selective Gelation in Composite PDMS Channels, RSC Advances, 9, 9136–9144 (2019).


H. Iwadate, M. Yamada, N. Kimura, R. Hashimoto, Y. Yajima, R. Utoh, and M. Seki: PDMS Microstencil Plate-supported Fabrication of Ultra-thin, Condensed ECM Membranes for Separated Cell Coculture on Both Surfaces, Sensors and Actuators B: Chemical, 287, 486-495 (2019).


S. Aoyama, Y. Akiyama, K. Monden, M. Yamada, and M. Seki: Thermally Imprinted Microcone Structure-assisted Lateral-flow Immunoassay Platforms for Detecting Disease Marker Proteins, Analyst, 144 (5), 1519-1526 (2019). (highlighted as outside cover)
  (linked to the website)

M. Sugimoto, Y. Kitagawa, M. Yamada, Y. Yajima, R. Utoh, and M. Seki: Micropassage-embedding Composite Hydrogel Fibers Enable Quantitative Evaluation of Cancer Cell Invasion under 3D Coculture Conditions, Lab on a Chip, 18 (9), 1378–1387 (2018).


M. Yamada and M. Seki: Multiphase Microfluidic Processes to Produce Alginate-based Microparticles and Fibers (Review), Journal of Chemical Engineering of Japan, 51 (4) 318-330 (2018).


Y. Yajima, C. N. Lee, M. Yamada, R. Utoh, and M. Seki: Development of a Perfusable 3D Liver Cell Cultivation System via Bundling-up Assembly of Cell-laden Microfibers, Journal of Bioscience and Bioengineering, 126 (1), 111–118 (2018).


R. Utoh, J. Komori, H. Kuge, K. Tatsumi, M. Yamada, S. Hirohashi, M. Tsutsumi, T. Amanuma, A. Yoshioka, Y. Nakajima, K. Wake, T. Okano, E. Lagasse, and K. Ohashi: Adult Hepatocytes Direct Liver Organogenesis through Non-parenchymal Cell Recruitment in the Kidney, Journal of Hepatology, 68 (4), 744–753 (2018).


K. Akamatsu, K. Minezaki, M. Yamada, M. Seki, and S. Nakao: Direct Observation of Splitting in Oil-in-water-in-oil Emulsion Droplets via a Microchannel Mimicking Membrane Pores, Langmuir, 33 (49), 14087–14092 (2017).


Y. Yajima, M. Yamada, R. Utoh, and M. Seki: Collagen Microparticle-mediated 3D Cell Organization: A Facile Route to Bottom-Up Engineering of Thick and Porous Tissues, ACS Biomaterials Science and Engineering, 3 (9), 2144-2154 (2017).


S. Tomii, M. Yamada, M. Mizuno, Y. Yamada, T. Kojima, M. Kushida, and M. Seki: Assembly of Carbon Nanotubes into Microparticles with Tunable Morphologies Using Droplets in a Non-equilibrium State, RSC Advances, 7 (29), 17773-17780 (2017).


M. Yamada, W. Seko, T. Yanai, K. Ninomiya, M. Seki: Slanted, Asymmetric Microfluidic Lattices as Size-selective Sieves for Continuous Particle/Cell Sorting, Lab on a Chip, 17 (2), 304-314 (2017).


K. Kinoshita, M. Iwase, M. Yamada, Y. Yajima, M. Seki: Fabrication of Multilayered Vascular Tissues Using Microfluidic Agarose Hydrogel Platforms, Biotechnology Journal, 11 (11), 1415-1423 (2016).


Y. Ichihara, R. Utoh, M. Yamada, T. Shimizu, Y. Uchigata: Size Effect of Engineered Islets Prepared Using Microfabricated Wells on Islet Cell Function and Arrangement, Heliyon, 2 (6), e00129. (2016).


N. Nakajima, M. Yamada, S. Kakegawa, M. Seki: Microfluidic System Enabling Multistep Tuning of Extraction Time Periods for Kinetic Analysis of Droplet-Based Liquid-Liquid Extraction, Analytical Chemistry, 88 (11), 5637-5643 (2016).


K. Endo, T. Anada, M. Yamada, M. Seki, K. Sasaki, O. Suzuki: Enhancement of Osteoblastic Differentiation in Alginate Gel Beads with Bioactive Octacalcium Phosphate Particles, Biomedical Materials, 10 (6), 065019 (2015).


M. Yamada, A. Hori, S. Sugaya, Y. Yajima, R. Utoh, M. Yamato, M. Seki: Cell-sized Condensed Collagen Microparticles for Preparing Microengineered Composite Spheroids of Primary Hepatocytes, Lab on a Chip, 15 (19), 3941–3951 (2015).


T. Sun, C. Z. Hu, M. Nakajima, M. Takeuchi, M. Seki, T. Yue, Q. Shi, T. Fukuda, Q. Huang: On‑chip Fabrication and Magnetic Force Estimation of Peapod‑like Hybrid Microfibers Using a Microfluidic Device, Microfluidics and Nanofluidics, 18 (5), 1177–1187 (2015).


M. Mizuno, T. Toyota, M. Konishi, Y. Kageyama, M. Yamada, M. Seki: Formation of Monodisperse Hierarchical Lipid Particles Utilizing Microfluidic Droplets in a Non-equilibrium State, Langmuir, 31 (8), 2334–2341 (2015).


Y. Kitagawa, Y. Naganuma, Y. Yajima, M. Yamada, M. Seki: Patterned Hydrogel Microfibers Prepared by Using Multilayered Microfluidic Devices for Guiding Network Formation of Neural Cells, Biofabrication, 6 (3), 035011 (2014).


Y. Yajima, M. Yamada, E. Yamada, M. Iwase, M. Seki: Facile Fabrication Processes for Hydrogel-based Microfluidic Devices Made of Natural Biopolymers, Biomicrofluidics, 8 (2), 024115 (2014).


T. Ono, M. Yamada, Y. Suzuki, T. Taniguchi, M. Seki: One-step Synthesis of Spherical/nonspherical Polymeric Microparticles Using Non-equilibrium Microfluidic Droplets, RSC Advances, 4 (24), 13557–13564 (2014).


H. Nabesawa, T. Hiruma, T. Hitobo, S. Wakabayashi, T. Asaji, T. Abe, M. Seki: Low-pressure Plasma-etching of Bulk Polymer Materials Using Gas Mixture of CF4 and O2, AIP Advances, 3, 112105 (2013).


S. Sugaya, M. Yamada, A. Hori, M. Seki: Microfluidic Production of Single Micrometer-sized Hydrogel Beads Utilizing Droplet Dissolution in a Polar Solvent, Biomicrofluidics, 7 (5), 054120 (2013).


M. Mizuno, M. Yamada, R. Mitamura, K. Ike, K. Toyama, M. Seki: Magnetophoresis-integrated Hydrodynamic Filtration System for Size- and Surface Marker-based Two-dimensional Cell Sorting, Analytical Chemistry, 85 (16), 7666–7673 (2013).


A. Kobayashi, K. Yamakoshi, Y. Yajima, R. Utoh, M. Yamada, M. Seki: Preparation of Stripe-patterned Heterogeneous Hydrogel Sheets Using Microfluidic Devices for High-density Coculture of Hepatocytes and Fibroblasts, Journal of Bioscience and Bioengineering, 116 (6), 761–767 (2013).


A. Miyama, M. Yamada, S. Sugaya, M. Seki: A Droplet-based Microfluidic Process to Produce Yarn-ball-shaped Hydrogel Microbeads, RSC Advances, 3 (30), 12299–12306 (2013).


M. Iwase, M. Yamada, E. Yamada, M. Seki: Formation of Cell Aggregates Using Microfabricated Hydrogel Chambers for Assembly into Large Tissues, Journal of Robotics and Mechatronics, 25 (4), 682–689 (2013).


K. Ohara, M. Kojima, A. Fukushima, S. Onozaki, M. Horade, M. Yamada, M. Seki, Y. Mae, and T. Arai: Automated Construction System for 3D Lattice Structure Based on Alginate Gel Fiber Containing Living Cells, Journal of Robotics and Mechatronics, 25 (4), 665–672 (2013).


T. Morijiri, M. Yamada, T. Hikida, M. Seki: Microfluidic Counterflow Centrifugal Elutriation System for Sedimentation-based Cell Separation, Microfluidics and Nanofluidics, 14 (6), 1049–1057 (2013).


S. Sugaya, S. Kakegawa, S. Fukushima, M. Yamada, M. Seki: Micropatterning of Hydrogels on Locally Hydrophilized Regions on PDMS by Stepwise Solution Dipping and in situ Gelation, Langmuir, 28 (39) 14073-14080 (2012).


M. Yamada, R. Utoh, K. Ohashi, K. Tatsumi, M. Yamato, T. Okano, M. Seki: Controlled Formation of Heterotypic Hepatic Micro-Organoids in Anisotropic Hydrogel Microfibers for Long-Term Preservation of Liver-Specific Functions, Biomaterials, 33 (33), 8304-8315 (2012).


K. Toyama, M. Yamada, M. Seki: Isolation of Cell Nuclei in Microchannels by Short-term Chemical Treatment via Two-step Carrier Medium Exchange, Biomedical Microdevices, 14 (4), 751-757 (2012).


M. Yamada, S. Sugaya, Y. Naganuma, M. Seki: Microfluidic Synthesis of Chemically and Physically Anisotropic Hydrogel Microfibers for Guided Cell Growth and Networking, Soft Matter, 8 (11), 3122-3130 (2012).


M. Masubuchi, T. Toyota, M. Yamada, M. Seki: Fluidic Shear-Assisted Formation of Actuating Multilamellar Lipid Tubes Using Microfabricated Nozzle Array Device, Chemical Communications, 47 (29), 8433-8435 (2011).


T. Moritani, M. Yamada, M. Seki: Generation of Uniform-size Droplets by Multistep Hydrodynamic Droplet Division in Microfluidic Circuits, Microfluidics and Nanofluidics, 11 (5), 601-610 (2011).


S. Sugaya, M. Yamada, M. Seki: Observation of Non-spherical Particle Behaviors for Continuous Shape-based Separation Using Hydrodynamic Filtration, Biomicrofluidics, 5 (2), 024103 (2011).


T. Morijiri, S. Sunahiro, M. Senaha, M. Yamada, M. Seki: Sedimentation Pinched-flow Fractionation for Size- and Density-based Particle Sorting in Microchannels, Microfluidics and Nanofluidics, 11 (1), 105-110 (2011).


Y. Suzuki, M. Yamada, M. Seki: Sol-gel Based Fabrication of Hybrid Microfluidic Devices Composed of PDMS and Thermoplastic Substrates, Sensors and Actuators B: Chemical, 148 (1), 323-329 (2010).


M. Yamada, P. Mao, J. Fu, J. Han: Rapid Quantification of Disease-marker Proteins Using Continuous-flow Immunoseparation in a Nano-sieve Fluidic Device, Analytical Chemistry, 81 (16), 7067-7074 (2009).


R. Aoki, M. Yamada, M. Yasuda, M. Seki: In-channel Focusing of Flowing Microparticles Utilizing Hydrodynamic Filtration, Microfluidics and Nanofluidics, 6 (4), 571-576 (2009).


K. Sawada, A. Taki, T. Yamakawa, M. Seki: Key Role for Transketolase Activity in Erythritol Production by Trichosporonoides Megachiliensis SN-G42., Journal of Bioscience and Bioengineering, 108 (5), 385-90 (2009).


S. Wakabayashi, T. Hitobo, H. Nabesawa, M. Seki: Microfabrication of Transparent Thermoplastic Resin Plates by Dry Etching, Journal of Vacuum Society of Japan, 52 (3), 138-140 (2009).


H. Nabesawa, T. Hitobo, S. Wakabayashi, T. Asaji, T. Abe, M. Seki: Polymer Surface Morphology Control by Reactive Ion Etching for Microfluidic Devices, Sensors and Actuators B: Chemical, 132 (2), 637-643 (2008).


M. Yamamoto, M. Yamada, N. Nonaka, S. Fukushima, M. Yasuda, M. Seki: Patterning Reactive Microdomains inside PDMS Microchannels by Trapping and Melting Functional Polymer Particles, Journal of the American Chemical Society, 130 (43), 14044-14045 (2008).


M, Matsuda, M. Yamada, M. Seki: Blood Cell Classification Utilizing Hydrodynamic Filtration, IEEJ Trans. Sens. Micro., 128-E (10), 396-401 (2008)


M. Yamada, J. Kobayashi, M.Yamato, M. Seki, T. Okano: Millisecond Treatment of Cells Using Microfluidic Devices via Two-step Carrier-medium Exchange, Lab on a Chip, 8 (5), 772-778 (2008)



M. Yamada, S. Doi, H. Maenaka, M. Yasuda, M. Seki: Hydrodynamic Control of Droplet Division in Bifurcating Microchannel and Its Application for Particles Synthesis, J. Colloid Interf. Sci., 321 (2), 401-407 (2008).


H. Maenaka, M. Yamada, M. Yasuda, M. Seki: Continuous and Size-dependent Sorting of Emulsion Droplets Using Hydrodynamics in Pinched Microchannels, Langmuir, 24 (8), 4405-4410 (2008).


T. Kawamata, M. Yamada, M. Yasuda, M. Seki : Continuous and Precise Particle Separation by Electroosmotic Flow Control in Microfluidic Devices, Electrophoresis, 29 (7), 1423-1430 (2008).


K. Miyanaga, M. Yamada. M. Seki: Development of a Micro-scale Cell Culture System for Strawberry Using Microfabricated Devices, Funct. Plant Sci. Biotechnol., 1 (2), 355-360 (2007).


F. Q. Nie, M. Yamada, J. Kobayashi, M. Yamato, A. Kikuchi, T. Okano: On-chip Cell Migration Assay Using Microfluidic Channels, Biomaterials, 28 (27), 4017-4022 (2007).


M. Yamada, K. Kano, Y. Tsuda, J. Kobayashi, M. Yamato, M. Seki, T. Okano: Microfluidic Devices for Size-dependent Separation of Liver Cells, Biomed. Microdev., 9 (5), 637-645 (2007).


C. Sakamoto, N. Yamaguchi, M. Yamada, H. Nagase, M. Seki, M. Nasu: Rapid Quantification of Bacterial Cells in Potable Water Using a Simplified Microfluidic Device, J. Microbiol. Methods, 68 (3), 643-647 (2007).


Y. Sai, M. Yamada, M. Yasuda, M. Seki: Continuous Separation of Particles Using a Microfluidic Device Equipped with Flow Rate Control Valves, J. Chromatogr. A, 1127 (1-2), 214-220 (2006).


M. Yamada, T. Hirano, M. Yasuda, M. Seki: A Microfluidic Flow Distributor Generating Stepwise Concentrations for High-throughput Biochemical Processing, Lab Chip, 6 (2), 179-184 (2006).


M. Yamada and M. Seki: Microfluidic Particle Sorter Employing Flow Splitting and Recombining, Anal. Chem., 78 (4), 1357-1362 (2006).


J. Edahiro, and M. Seki: Phenylpropanoid metabolite supports cell aggregate formation in strawberry cell suspension culture, J. Biosci. Bioeng., 102 (1), 8-13, (2006).


M. Yamada and M. Seki: Hydrodynamic Filtration for On-chip Particle Concentration and Classification Utilizing Microfluidics, Lab Chip, 5 (11), 1233-1239 (2005).


N. Y. Lee, M. Yamada, M. Seki: Development of a Passive Micromixer Adopting Repeated Fluid Twisting and Flattening, and Its Application to DNA Purification, Anal. Bioanal. Chem., 383 (5), 776-782 (2005).


J. Edahiro, M. Yamada, S. Seike, Y. Kakigi, K. Miyanaga, M. Nakamura, T. Kanamori, M. Seki: Separation of Cultured Strawberry Cells Producing Anthocyanins in Aqueous Two-Phase System, J. Biosci. Bioeng., 100 (4), 449-454 (2005).


J.Takagi, M. Yamada, M. Yasuda, M. Seki: Continuous Particle Separation in a Microchannel Having Asymmetrically-Arranged Multiple Branches, Lab Chip, 5 (7), 778-784 (2005).


N. Y. Lee, M. Yamada, M. Seki: Control-free Air Vent System for Ultra-low Volume Sample Injection on a Microfabricated Device, Anal. Sci., 21 (4), 465-468 (2005).


H. Ogino, T. Miura, K. Ishimi, M. Seki, H. Yoshida: Hydrogen Production from Glucose by Anaerobes, Biotechnol. Prog., 21 (6), 1786-1788 (2005).


J. Edahiro, M. Nakamura, M. Seki, S. Furusaki: Enhanced accumulation of anthocyanin in cultured strawberry cells by repetitive feeding of L-phenylalanine into the medium, J. Biosci. Bioeng., 99 (1), 43-47 (2005).


M. Yamada, V. Kasim, M. Nakashima, J. Edahiro, M. Seki: Continuous Cell Partitioning Using an Aqueous Two-phase Flow System in Microfluidic Devices, Biotechnol. Bioeng., 88 (4), 489-494 (2004).


M. Yamada, M. Nakashima, M. Seki: Pinched Flow Fractionation: Continuous Size Separation of Particles Utilizing a Laminar Flow Profile in a Pinched Microchannel, Anal. Chem., 76 (18), 5465-5471 (2004).


N. Y. Lee, M. Yamada, M. Seki: Pressure-driven Sample Injection with Quantitative Liquid Dispensing for On-chip Electrophoresis, Anal. Sci., 20 (3), 483-487 (2004).


M. Yamada and M. Seki: Nanoliter-sized Liquid Dispenser Array for Multiple Biochemical Analysis in Microfluidic Devices, Anal. Chem., 76 (4), 895-899 (2004).


K. Koyama, M. Seki: Evaluation of mass-transfer characteristics in alginate-membrane liquid-core capsules prepared using polyethylene glycol, J. Biosci. Bioeng., 98 (2), 114-21 (2004).


K. Koyama, M. Seki: Cultivation of yeast and plant cells entrapped in the low-viscous liquid-core of an alginate membrane capsule prepared using polyethylene glycol, J. Biosci. Bioeng., 97 (2), 111-8 (2004).


S. Sugiura, M. Nakajima, M. Seki: Prediction of droplet diameter for microchannel emulsification: Prediction model for complicated microchannel geometries, Ind. Eng. Chem. Res., 43 (26), 8233-8238 (2004).


S. Sugiura, M. Nakajima, K. Yamamoto, S. Iwamoto, T. Oda, M. Satake, M. Seki: Preparation characteristics of water-in-oil-in-water multiple emulsions using microchannel emulsification, J. Colloid Interf. Sci., 270 (1), 221-8 (2004).


S. Sugiura, M. Nakajima, T. Oda, M. Satake, M. Seki: Effect of interfacial tension on the dynamic behavior of droplet formation during microchannel emulsification, J. Colloid Interf. Sci., 269 (1), 178-85 (2004).


M. Seki: Microfluidic Devices and Micro-bioreactor, Nippon Nogeikagaku Kaishi, 77 (9), 865-867 (2003).


T. Futami, M. Miyagishi, S. Iwai, M. Seki, K. Taira: Stimulatory effect of an indirectly attached RNA helicase-recruiting sequence on the suppression of gene expression by antisense oligonucleotides, Antisense Nucleic Acid Drug Dev., 13 (1), 9-17 (2003).


T. Maruyama, M. Nakajima, H. Kondo, K. Kawasaki, M Seki, M. Goto: Can lipases hydrolyze a peptide bond?, Enzyme Microb. Technol., 32 (6), 655-657 (2003).


T. Maruyama, S. Umezaki, M. Nakajima, M. Seki: Interesterification and hydrolysis catalyzed by fatty acid-modified lipases, Eur. J. Lipid Sci. Technol., 104 (5), 255-261, (2002).


H. Akashi, H. Kurata, M. Seki, K. Taira, S. Furusaki: Screening for transgenic plant cells that highly express a target gene from genetically mixed cells, Biochem. Eng. J., 10 (3), 175-182, (2002).


T. Futami, M. Miyagishi, M. Seki, K. Taira: Induction of apoptosis in HeLa cells with siRNA expression vector targeted against bcl-2, Nucleic Acids Res Suppl., (2), 251-252, (2002).


K. Matsumoto, H. Matsusaki, K. Taguchi, M. Seki, Y. Doi: Isolation and characterization of polyhydroxyalkanoates inclusions and their associated proteins in Pseudomonas sp. 61-3., Biomacromolecules., 3 (4), 787-92 (2002).


S. Sugiura, M. Nakajima, N. Kumazawa, S. Iwamoto, M. Seki: Characterization of spontaneous transformation-based droplet formation during microchannel emulsification, J. Phys. Chem. B, 106 (36), 9405-9409, (2002).


S. Sugiura, M. Nakajima, M. Seki: Preparation of monodispersed polymeric microspheres over 50 μm employing microchannel emulsification, Ind. Eng. Chem. Res., 41 (16), 4043-4047, (2002).


S. Sugiura, M. Nakajima, M. Seki: Effect of channel structure on microchannel emulsification, Langmuir, 18 (15), 5708-5712 (2002).


S. Sugiura, M. Nakajima, M. Seki: Prediction of droplet diameter for microchannel emulsification, Langmuir, 18 (10), 3854-3859 (2002).


S. Sugiura, M. Nakajima, M. Seki: Preparation of monodispersed emulsion with large droplets using microchannel emulsification, J. Am. Oil Chem. Soc., 79 (5), 515-519, (2002).


K. Matsumoto, S. Nakae, K. Taguchi, H. Matsusaki, M. Seki, Y. Doi: Poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) copolymer from sugars by recombinant Ralstonia eutropha harboring the phaC1Ps and the phaGPs genes of Pseudomonas sp. 61-3., Biomacromolecules, 2 (3), 934-939 (2001).


K. Matsumoto, H. Matsusaki, S. Taguchi, M. Seki, Y. Doi: Cloning and characterization of the pseudomonas sp. 61-3phaG gene involved in polyhydroxyalkanoate biosynthesis, Biomacromolecules, 2 (1), 142-147 (2001).


S. Sugiura, M. Nakajima, S. Iwamoto, M. Seki: Interfacial tension driven monodispersed droplet formation from microfabricated channel array, Langmuir, 17 (18), 5562-5566 (2001).


S. Sugiura, M. Nakajima, H. Itou, M. Seki: Synthesis of polymeric microspheres with narrow size distributions employing microchannel emulsification, Macromol. Rapid Commun., 22 (10), 773-778 (2001).


S. Sugiura, M. Nakajima, H. Ushijima, K. Yamamoto, M. Seki: Preparation characteristics of monodispersed water-in-oil emulsions using microchannel emulsification, J. Chem. Eng. Jpn., 34 (6), 757-765 (2001).


S. Sugiura, S. Ichikawa, Y. Sano, M. Nakajima, XQ. Liu, M. Seki, S. Furusaki: Formation and Characterization of Reversed Micelles Composed of Phospholipids and Fatty Acids, J. Colloid Interf. Sci., 240 (2), 566-572 (2001).


J. W. Hong, K. Hosokawa, T. Fujii, M. Seki, I. Endo: Microfabricated polymer chip for capillary gel electrophoresis, Biotechnol. Prog., 17 (5), 958-962 (2001).


J. W. Hong, T. Fujii, M. Seki, T. Yamamoto, I. Endo: Integration of gene amplification and capillary gel electrophoresis on a polydimethylsiloxane-glass hybrid microchip, Electrophoresis, 22 (2), 328-333 (2001).


T. Maruyama, M. Nakajima, S. Ichikawa, S. Furusaki, M. Seki: Structural study of lipase modified with fatty acids, Biochem. Eng. J., 9 (3), 185-191 (2001).


T. Maruyama, M. Nakajima, M .Seki: Effect of hydrocarbon-water interfaces on synthetic and hydrolytic activities of lipases, J. Biosci. Bioeng., 92 (3), 242-247 (2001).


T. Maruyama, M. Nakajima, S. Ichikawa, Y. Sano, H. Nabetani, S. Furusaki, M. Seki: Small-angle X-ray scattering analysis of stearic acid modified lipase, Biosci. Biotechnol. Biochem., 65 (4), 1003-1006 (2001).


S. Ichikawa, S. Sugiura, M. Nakajima, Y. Sano, M. Seki, S. Furusaki: Formation of biocompatible reversed micellar systems using phospholipids, Biochem. Eng. J., 6 (3), 193-199 (2000).


S. Sugiura, M. Nakajima, J. Tong, H. Nabetani, M. Seki: Preparation of monodispersed solid lipid microspheres using a microchannel emulsification technique, J. Colloid Interf. Sci., 227 (1), 95-103 (2000).


T. Maruyama, M. Nakajima, S. Ichikawa, H. Nabetani, S. Furusaki, M. Seki: Oil-water interfacial activation of lipase for interesterification of triglyceride and fatty acid, J. Am. Oil Chem. Soc., 77 (11), 1121-1126 (2000).


K. Miyanaga, M. Seki, S. Furusaki: Quantitative determination of cultured strawberry-cell heterogeneity by image analysis: Effects of medium modification on anthocyanin accumulation, Biochem. Eng. J., 5 (3), 201-207 (2000).


H. Kurata, A. Mochizuki, N. Okuda, M. Seki, S. Furusaki: Intermittent light irradiation with second- or hour-scale periods controls anthocyanin production by strawberry cells, Enzyme Microb. Technol., 26 (8), 621-629 (2000).


K. Miyanaga, M. Seki, S. Furusaki: Analysis of pigment accumulation heterogeneity in plant cell population by image-processing system, Biotechnol. Bioeng., 67 (4), 493-497 (2000).


K. Miyanaga, M. Seki, S. Furusaki: Analysis of pigmentation in individual cultured plant cells using an image processing system, Biotechnol. Lett., 22 (12), 977-981 (2000).


Y. Nara, H. Kurata, M. Seki, K. Taira: Glucocorticoid-induced expression of a foreign gene by the GVG system in transformed tobacco BY-2 cells, Biochem. Eng. J., 6 (3), 185-191 (2000).